Basic infomation

source

Mouse

Clonality

Polyclonal

Isotype

IgG

Reactivity

H M R

MW (kDa)

36kDa

Purification

Antigen affinity purfication

UniProt ID

P04406

NCBI Gene

2597

Applications

Tested Applications

WB,

Cited Applications

WB, IHC, IF

Product Usage

Information

Western Blotting

1:1000-1:5000

Immunohistochemistry

1:200-1:800

Immunofluorescence

1:200-1:800

Storage

PBS with 50% glycerol and less than 0.02% sodium azide

Store at –20°C, stable for one year after shipment

Do not aliquot the antibody

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Abbreviation

H: human M: mouse R: rat Rb: rabbit Hm: hamster Mk: monkey Vir: virus C: chicken  Z: zebrafish Dg: dog Pg: pig Hr: horse