Basic infomation

source

Mouse

Clonality

Polyclonal

Isotype

IgG

Reactivity

H M R

MW (kDa)

43kDa

Purification

Antigen affinity purfication

UniProt ID

P27361

NCBI Gene

5595

Applications

Tested Applications

WB,

Cited Applications

WB, IHC, IF

Product Usage

Information

Western Blotting

1:1000-1:2000

Immunohistochemistry

1:100-1:200

Immunofluorescence

1:100-1:200

Storage

PBS with 50% glycerol and less than 0.02% sodium azide

Store at –20°C, stable for one year after shipment

Do not aliquot the antibody

Background

The protein (ERK1) encoded by this gene (MAPK3) is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding ERK1 conjugated to KLH. Phosphopeptide containing human ERK1 T202/Y204site.

Abbreviation

H: human M: mouse R: rat Rb: rabbit Hm: hamster Mk: monkey Vir: virus C: chicken  Z: zebrafish Dg: dog Pg: pig Hr: horse